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Rev Esp Quimioter 2020; 33(4): 258-266

Utility of lateral flow tests in SARS-CoV-2 infection monitorization   

FRANCISCO JAVIER CANDEL GONZÁLEZ, JOSÉ MANUEL VIÑUELA-PRIETO, JUAN GONZÁLEZ DEL CASTILLO, PABLO BARREIRO GARCÍA, MARCOS FRAGIEL SAAVEDRA, ALBA HERNÁNDEZ PÍRIZ, DAVID JIMÉNEZ VIRUMBRALES, JESÚS CANORA LEBRATO, GONZALO GARCÍA DE CASASOLA, RUTH GIL PRIETO, JESÚS SAN-ROMÁN MONTERO, JUSTO ORTEGA ANSELMI, RAQUEL BARBA MARTÍN, FERNANDO PRADOS ROA, JAVIER MARCO MARTÍNEZ, ANTONIO ZAPATERO GAVIRIA

Published: 4 June 2020

http://www.doi.org/10.37201/req/052.2020

Introduction. The diagnosis of SARS-CoV-2 infection is crucial for medical and public health reasons, to allow the best treatment of cases and the best control of the pandemic. Serology testing allows for the detection of asymptomatic infections and 19-COVID cases once the virus has been cleared. We analyzed the usefulness of the SARS-CoV-2 rapid test of Autobio and tried to correlate its pattern with the severity of COVID19 infection.
Material and methods. We analyzed the accuracy and clinical usefulness of a point-of-care IgM and/or IgG test for SARS-CoV-2 in 35 COVID-19 patients [12 (34.3%) mild-moderate and 23 (65.7%) severe-critical] admitted to a field hospital in Madrid, as well as in 5 controls.
Results. The mean time from the first day of symptoms to the antibody test was 28 days (SD: 8.7), similar according to the severity of the disease. All patients with SARS-CoV-2 PCR+ showed the corresponding IgG positivity, while these results were negative in all control individuals. A total of 26 (74%) cases also presented with positive IgM, 19 (83%) were severe-critical cases and 7 (58%) were mild-moderate cases. The IgM response lasted longer in the severe critical cases (mean: 29.7 days; SD: 8.4) compared to the moderate cases (mean: 21.2 days; SD: 2.0).
Conclusions. Rapid serology tests are useful for the diagnosis of patients with COVID-19 (mainly IgG detection) and may also be correlated with the severity of the infection (based on IgM detection).

Rev Esp Quimioter 2020; 33(4): 258-266 [Full-text PDF]


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Rev Esp Quimioter 2020; 33(4): 278-280

Neumonía bilateral en un paciente con un síndrome de Cushing   

(Bilateral pneumonia in a patient with Cushing’s syndrome)

JUAN MONTE ARMENTEROS, ELENA BERECIARTUA BASTARRICA, UNAI JIMÉNEZ MAESTRE, LAURA GUÍO CARRIÓN

Published: 28 May 2020

http://www.doi.org/10.37201/req/001.2020

LETTER TO THE EDITOR

Rev Esp Quimioter 2020; 33(4): 278-280 [Texto completo PDF]


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Rev Esp Quimioter 2020; 33(4): 281-282

Infección urinaria por Acinetobacter dijkshoorniae y buena respuesta clínica al tratamiento   

(Urinary tract infection by Acinetobacter dijkshoorniae and good clinical response to treatment)

MARÍA ISABEL CASANOVAS MORENO-TORRES, FANNY RODRÍGUEZ-CAMPOS, MIGUEL GUTIÉRREZ-SOTO, JOSÉ MARÍA NAVARRO-MARÍ, JOSÉ GUTIÉRREZ-FERNÁNDEZ

Published: 28 May 2020

http://www.doi.org/10.37201/req/011.2020

LETTER TO THE EDITOR

Rev Esp Quimioter 2020; 33(4): 281-282 [Texto completo PDF]


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Rev Esp Quimioter 2020; 33(4): 292-293

Evaluación de diferentes genes en la detección por RT-PCR del SARS-CoV-2 en muestras respiratorias y su evolución en la infección   

(Evaluation of different genes in the RT-PCR detection of SARS-CoV-2 in respiratory samples and its evolution in infection)

JORDI REINA, LORETO SUAREZ

Published: 27 May 2020

http://www.doi.org/10.37201/req/045.2020

LETTER TO THE EDITOR

Rev Esp Quimioter 2020; 33(4): 292-293 [Texto completo PDF]


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Rev Esp Quimioter 2020; 33(4): 289-291

COVID-19 en pacientes españoles e inmigrantes en un área sanitaria de Madrid   

(COVID-19 in Spanish and immigrant patients in a sanitary district of Madrid)

JERÓNIMO JAQUETI AROCA, LAURA M. MOLINA ESTEBAN, ISABEL GARCÍA-ARATA, JESÚS GARCÍA-MARTÍNEZ

Published: 20 May 2020

http://www.doi.org/10.37201/req/041.2020

LETTER TO THE EDITOR

Rev Esp Quimioter 2020; 33(4): 289-291 [Texto completo PDF]


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Rev Esp Quimioter 2020; 33(3): 216-217

Listeria monocytogenes, a rare cause of endophthalmitis   

(Listeria monocytogenes, una rara causa de endoftalmitis)

LINA MARTIN HITA, ISABEL CASANOVAS MORENO- TORRE, JAIME BORREGO JIMÉNEZ, CARLA FORONDA GARCÍA- HIDALGO, VICENTE GUILLOT SUAY, JOSE MARÍA NAVARRO MARI

Published: 13 May 2020

http://www.doi.org/10.37201/req/097.2019

LETTER TO THE EDITOR

Rev Esp Quimioter 2020; 33(3): 216-217 [Full-text PDF]


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Rev Esp Quimioter 2020; 33(3): 193-199

Evaluation of Sysmex UF-1000i® flow cytometer as a screening method for asymptomatic bacteriuria and detection of Group B Streptococcus in pregnancy  

ITZIAR ANGULO LÓPEZ, MIKEL URRUTIKOETXEA-GUTIÉRREZ, JULIA ARAGÓN-DÍEZ, MERCEDES FRACA PADILLA, JOSÉ LUIS DÍAZ DE TUESTA DEL ARCO

Published: 11 May 2020

http://www.doi.org/10.37201/req/017.2020

Objectives. Nowadays, the use of flow-cytometry for the screening of urine samples is extended, but appropriate cut-off points for each population group are yet to be established. The objective of this study was to evaluate the Sysmex UF-1000i® cytometer as a screening method for detection of asymptomatic bacteriuria (AB) and Group B Streptococcus (GBS) in pregnant women.
Material and methods. Urine samples obtained during pregnancy between January-July 2019 were both processed with the Sysmex UF-1000i® and also cultured. Demographic data, flow-cytometry parameters and the result of the urine culture were collected. To assess the performance of the flow-cytometer for detection of AB and GBS, receiver operating characteristic (ROC) curves for the BACT/μL variable were applied.
Results. A total of 33,687 urine samples were received, among which 1,443 (4.3%) belonged to pregnant women. Urine culture was positive in 82 (5.7%) samples, 1,295 (89.7%) were negative and 66 (4.6%) were considered contaminated. GBS was isolated in 69 (4.8%) patients and 46 (66.7%) with a microbial count below 10E4 CFU/mL. For AB detection, the cut-off point of 550 BACT/μL yielded a sensitivity of 91.5%, a negative predictive value of 99.3% and could avoid culturing 74.1% of the samples. No cut-off value could be established for GBS detection.
Conclusions. Although the Sysmex UF-1000i® system is a valid screening method for the AB detection in pregnant population, it is not useful for the identification of GBS bacteriuria in our area. Therefore, the conventional urine culture is still required during pregnancy.

Rev Esp Quimioter 2020; 33(3): 193-199 [Texto completo PDF]


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Rev Esp Quimioter 2020; 33(3): 200-206

Etiology of bloodstream infections at a population level during 2013-2017 in the Autonomous Community of Valencia, Spain   

LAURA RUIZ-AZCONA, MIGUEL SANTIBAÑEZ, ADELINA GIMENO, FRANCISCO JAVIER ROIG, HERMELINDA VANACLOCHA, MARIA PAZ VENTERO, VICENTE BOIX, JOSÉ SÁNCHEZ-PAYÁ, JOAQUÍN PORTILLA-SOGORB, ESPERANZA MERINO, JUAN CARLOS RODRÍGUEZ

Published: 29 April 2020

http://www.doi.org/10.37201/req/024.2020

Introduction. Bloodstream Infections has become in one of the priorities for the antimicrobial stewardship teams due to their high mortality and morbidity rates. Usually, the first antibiotic treatment for this pathology must be empirical, without microbiology data about the microorganism involved. For this reason, the population studies about the etiology of  bacteremia are a key factor to improve the selection of the empirical treatment, because they describe the main microorganisms associated to this pathology in each area, and this data could facilitate the selection of correct antibiotic therapy.
Material and methods. This study describes the etiology of bloodstream infections in the Southeast of Spain. The etiology of bacteremia was analysed by a retrospective review of all age-ranged patients from every public hospital in the Autonomous Community of Valencia (approximately 5,000,000 inhabitants) for five years.
Results. A total of 92,097 isolates were obtained, 44.5% of them were coagulase-negative staphylococci. Enterobacteriales was the most prevalent group and an increase in frequency was observed along the time. Streptococcus spp. were the second microorganisms more frequently isolated. Next, the most prevalent were Staphylococcus aureus and Enterococcus spp., both with a stable incidence along the study. Finally, Pseudomonas aeruginosa was the fifth microorganism more frequently solated.
Conclusions. These data constitute a useful tool that can help in the choice of empirical treatment for bloodstream infections, since the knowledge of local epidemiology is key to prescribe a fast and appropriate antibiotic therapy, aspect capital to improve survival.

Rev Esp Quimioter 2020; 33(3): 200-206 [Full-text PDF]


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Rev Esp Quimioter 2020; 33(3): 223-224

Identificación de Brucella melitensis como Ochrobactrum anthropi mediante MALDI-TOF MS   

(Identification of Brucella melitensis as Ochrobactrum anthropi by MALDI-TOF MS)

TATIANA KHALIULINA USHAKOVA, ANA ISABEL PERERA LERIN, JUAN SAHAGÚN PAREJA, RAQUEL DOLZ ASPAS, MARTA PUYAL BARCELONA, ALEJANDRA SANCHO GARCÍA, BEATRIZ JIMÉNEZ MORALEDA, CRISTINA MATOVELLE OCHOA, SOLEDAD SALVO GONZALO

Published: 20 April 2020

http://www.doi.org/10.37201/req/009.2020

LETTER TO THE EDITOR

Rev Esp Quimioter 2020; 33(3): 223-224 [Texto completo PDF]


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Rev Esp Quimioter 2020; 33(3): 187-192

Epidemiology and etiology of vulvovaginal candidiasis in Spanish and immigrants’ women in Fuenlabrada (Madrid)   

JERÓNIMO JAQUETI AROCA, PAULA RAMIRO MARTÍNEZ, LAURA M MOLINA ESTEBAN, ALBA M. FERNÁNDEZ GONZÁLEZ, ISABEL GARCÍA-ARATA, SANTIAGO PRIETO MENCHERO

Published: 17 April 2020

http://www.doi.org/10.37201/req/099.2019

Objectives. Vulvovaginal candidiasis (VVC) is a common vaginal infection. Risk factors include diabetes, antibiotic use and pregnancy. Candida albicans is the most common species identified but non-C. albicans species appear to be more commonly associated with VVC in some Asian and African countries. We had studied the distribution of Candida species in Spanish and immigrants’ women residents in Spain.
Material and methods. Retrospective study of vaginal yeast cultures between 2015 and 2018.
Results. A total of 2,283 vaginal yeast cultures were collected. Candida spp. was detected in 25.7% from Spanish women and in 28.5% from immigrants (no significant differences). Immigrants have higher rates of vaginal candidiasis compared other studies in Spain. C. albicans was the most common species isolated (82.4%).
Conclusions. There were no differences in vaginal candidiasis rate between Spanish and immigrants’ women. Immigrants consulted proportionally more compared with the Spanish women.

Rev Esp Quimioter 2020; 33(3): 187-192 [Texto completo PDF]


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