Rev Esp Quimioter 2018; 31(1): 21-26
Prevalence and phylogenetic analysis of Chlamydia trachomatis in a population of women in Posadas, Misiones
GRACIELA BEATRIZ JORDÁ, SILVINA ELIZABETH HANKE, JOSÉ MANUEL RAMOS-RINCÓN, JESSICA MOSMANN, MARÍA LORENA LOPÉZ, ANDREA CAROLINA ENTROCASSI, CECILIA CUFFINI
Background. Chlamydia trachomatis is the most prevalent bacteria causing sexually transmitted infections. In women, this infection can cause cervicitis and urethritis, although it’s usually asymptomatic. The aim of this study was to investigate the prevalence of C. trachomatis in women attending the lab Instituto de Previsión Social and detect the genotypes.
Material and methods. Endocervical samples from 505 symptomatic and asymptomatic women were assayed. It was determined the presence of C. trachomatis by PCR through amplification of a fragment of the cryptic plasmid. Positive samples were genotyped by the partial amplification of the ompA gene and analyzed phylogenetically.
Results. Forty-three positive samples were detected to infection with C. trachomatis, obtaining a prevalence of 8.5% (IC 95%: 6.4-11.3%). The prevalence of C. trachomatis was higher in women with vaginal symptoms [11.3% (30/265) vs. 5.4% (13/240)] (p = 0.018), as well as in women under 26 year-old [11.5% (28/244) vs. 6.2% (15/246)] (p = 0.021). Based on phylogenetic analysis, it was observed that 62% of the samples were genotype E, 15% genotype J, 15% genotype D, and 8% genotype F.
Conclusions. This work is the first contribution on the molecular epidemiology of C. trachomatis in the Misiones province, Argentina, which shows the rate of prevalence of this bacterium and offers information on circulating genotypes.
Rev Esp Quimioter 2017; 31(1): 21-26 [Texto completo PDF]