Rev Esp Quimioter 2024, September 19
A 5-year study of bloodstream infections caused by carbapenemase-producing Gram-negative bacilli in southern Spain
FERNANDO COBO, JUAN ANTONIO REGUERA-MÁRQUEZ, JOSÉ ANTONIO MARÍN-RODRÍGUEZ, FRANCISCO JOSÉ MARTÍN-PÉREZ, PATRICIA PÉREZ-PALACIOS, ESTHER RECACHA, JOSÉ MARÍA NAVARRO-MARÍ
Published: 19 September 2024
http://www.doi.org/10.37201/req/045.2024
Introduction. The aim of this study was to evaluate the microbiological epidemiology of carbapenemase-producing Gram-negative bacilli (CPGNB) isolated from blood during a 5-year period.
Methods. A total of 80 isolates from 78 patients were finally included; fifty-five (70.5%) were men and the mean age was 60 years. Detection of carbapenemase production was
performed by immunocromatography (IC) and polymerase chain reaction (PCR). Genotyping was carried-out by pulsedfield gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), and characterization of carbapenemase-producing isolates was performed by whole genome sequencing (WGS).
Results. The main microorganisms isolated were K. pneumoniae (29.4%), E. cloacae (28.2%), A. baumannii (17.9%) and P. aeruginosa (15.3%). Overall, the most common carbapenemase in Enterobacterales was OXA-48 group (57.7%). The most common carbapenemase in non-fermenting bacilli was OXA-23 (60.8%). The most common ST in K. pneumoniae producing OXA-48 types was ST45 and in E. cloacae ST114, while in E. cloacae producing VIM types was ST78. In OXA-23 types, the most common clone in A. baumannii was ST2, whereas in P. aeruginosa producing IMP types was ST253.
Conclusions. There was an increase in cases recorded in the years of highest incidence and severity of the SARS-CoV-2 pandemic, with a significant number of cases in patients admitted to the ICU. All bacteremias caused by A. baumannii were caused by the same clone, and 12 of the 14 cases caused by A. baumannii were part of outbreaks in the ICU.
Rev Esp Quimioter 2024; September 19 [Full-text PDF]
